Content (Syllabus outline)

Concepts and principles of analytical approaches. The importance and methods of sampling, the use of control elements. Detection limits and errors in the analytical procedures. Presentation of the relevant chemical, physical and molecular biological analytical methods used in microbiology. Spectrophotometry and various types of spectrophotometric analysis. Applied aspects of spectrophotometric analysis (UV / VIS, fluorescence, FTIR, CD and others). Molecular biological methods useful in microbial ecological experiments and sanitary medical microbiology and biotechnology (typing methods, quantification methods. Review of available commercial kits for rapid and automated microbiological analysis. Flow cytometry and its application in microbiology. Principles of the radioisotope material usage in microbiological experiments. Fundamentals of gas-chromatographic analytical (GC) and liquid chromatography at high and medium pressure (MPLC and HPLC). Instrumental equipment and the use of comparative aspects: clinical diagnosis of pathogenic microbes, food and pharmaceutical industry, biotechnology and nature conservation research. Fundamentals of thin layer chromatography. Biosensors - the use of microorganisms as analytical tools. Standardization and the use of standardized methods in microbiology.

Prerequisites

a. Entry in the 3rd Year of the first Level program
b. Delivered seminar and passed colloquium are prerequisites for the approach to the final exam.