Development of protein analysis and purification methods (various types of fractionation, analytical and preparative chromatography, SDS-PAGE, WB), characterization of protein interactions, enzyme kinetics, detection and manipulation of DNA (protein engineering, site-specific mutagenesis, molecular cloning), expression of recombinant proteins in bacterial cells and refolding, confocal microscopy.

Research Fields

Bioactive milk protein and bacteriocin purification and analytics.

Selected Bibliography

  • OBERČKAL, Jernej, BOGOVIČ MATIJAŠIĆ, Bojana, ZUPANČIČ JUSTIN, Maja. Isolation of osteopontin and glycomacropeptide from whey : application no. št. LU502808, application date 16. 9. 2022. Luxembourg: Le Gouvernement du Grand-Duché, Office de la propriété intellectuelle, 2022. [22] str. [COBISS.SI-ID 128433155]
  • OBERČKAL, Jernej, LIAQAT, Humna, BOGOVIČ MATIJAŠIĆ, Bojana, ROZMAN, Vita, TREVEN, Primož. Quantification of lactoferrin in human milk using monolithic cation exchange HPLC. Journal of chromatography. B., Analytical technologies in the biomedical and life sciences. 2023, vol. 1214, art. 123548, str. 1-7, ilustr. ISSN 1873-376X., DOI: 10.1016/j.jchromb.2022.123548. [COBISS.SI-ID 132227587]
  • BOGOVIČ MATIJAŠIĆ, Bojana, OBERČKAL, Jernej, MOHAR LORBEG, Petra, PAVELJŠEK, Diana, SKALE, Nina, KOLENC, Borut, GRUDEN, Špela, POKLAR ULRIH, Nataša, KETE, Marko, ZUPANČIČ JUSTIN, Maja. Characterisation of lactoferrin isolated from acid whey using pilot-scale monolithic ion-exchange Chromatography. Processes. [Online ed.]. 2020, vol. 8, art. no. 804, str. 1-19, ilustr. ISSN 2227-9717., DOI: 10.3390/pr8070804. [COBISS.SI-ID 23698947]