Content (Syllabus outline)

  • Major metabolic pathways of primary metabolism: glycolysis, pentose phosphate pathway, TCA cycle, OXPHOS
  • Regulation of primary metabolism
  • Metabolic engineering: Metabolic flow analysis (MFA), metabolic control analysis (MCA)
  • Anaplerosis- cathaplerosis
  • Major anaplerotic reactions in the cells
  • Posttranslational modification of 6-phosphofructo-1-kinase (PFK1) as an important anaplerotic reaction
  • Construction of a modified gene encoding highly active short PFK1 fragment
  • Posttranslational modification of human PFK-M enzyme as an important feature of cancer metabolism

 

  • Secondary metabolism – definition

 

  • Secondary metabolites – definition

 

  • Biotechnological value of secondary metabolites

 

  • Precursors for secondary metabolites

 

  • Influence of primary metabolites on the production of secondary metabolites

 

  • Regulation of secondary metabolism

 

           Genetic mechanisms for the improvement of secondary metabolism

           Recombinant DNA technology: increasing gene copy number, use of strong promoters, removal of regulatory proteins, deletion of the genes encoding other secondary metabolites, insertion of heterologous genes or gene clusters

           Modern techniques: systems biology, metabolic engineering, directed evolution, molecular breeding techniques, combinatorial biosynthesis

           Induction of specific signaling pathways

           Endogenous or exogenous induction of signaling pathways

  • Technology of heterologous protein production

          Deletion of proteases in commercial microorganisms

          Molecular biology of protein secretory pathways

Prerequisites

General conditions for PhD study application