Content (Syllabus outline)
The basic processes of creation and the legal protection of plant varieties. Application of plant tissue culture methods in genetics and plant breeding. Basic approach to induction of haploids in plants. Methods of isolation and regeneration of protoplasts. Genetic transformations: basic techniques of indirect and direct gene transfer and analysis of transient and stable transformation. Genetic modification of agronomic traits: breeding of genetically modified plants with resistance to phytopathogenic viruses, bacteria and fungi and pests; GM plants with resistance to low and high temperature, salinity and GM plants with tolerance to herbicides. Genetic modifications of yield quality: improvement of nutritional value, reduction of allergens, increased value of health beneficial substances. GM plants for the production of antibodies, vaccines, drugs and biomaterials.
Development of molecular markers (AFLP, SSR, SNP, ITS) and evaluation of polymorphism with fenetic and model-based methods for the analysis of genomic diversity of plant species and populations. Application of molecular markers for gene mapping (recombinant maps), for isolation of candidate genes (positional cloning) and for searching for markers linked to important traits (segregation analysis). Molecular markers for the identification of crop varieties and for the diagnostics of plant pathogens. Introduction to plant genomics.
Prerequisites
Requirements for enrolment;
Enrolment in the 3rd year of the study program.
Terms of Prerequisites:
No prerequisites.