Content (Syllabus outline)
The principles of enzyme use in industry and at home are explained during the lectures and only selected examples are described. The whole breadth of enzyme use is covered by students’ essays.
We begin with the rationale for enzyme usage in industry and the fields of their use. The role of applied enzymatics in sustained development is highlited. The way the enzyme process is developed (and the process window defined) is explained. Peculiarities of enzyme use in idustry are explained:
“reverse” reactions, equilibrium reactions, kinetically controlled reactions: selectivity, specificity, stereospecificity. End point of enzyme process: »space time yield«. Enzyme sources, production, purity, stability, safety considerations. The use of enzymes with slightly soluble substrates, in suspensions or organic solvents. The use of soluble enzymes and the strengths and weaknesses of this approach are described using the example of starch degradarion. Enzyme improvement: in vitro evolution, recombination and rational approach: targeted mutagenesis and protein engineering
Comparision of rational approach and in vitro evolution is presented. The metagenomic screening for novel enzymes.
Benefits of membrane enzyme processes and immobilized enzymes are described. Also the carriers and ways of immobilization are discussed.
The detailed description of the semisynthetic pennicilin and cephalosporin enzyme process development is given: the enzyme properties, side reactions, decay of products during the process. The main types of reactor used in applied enzymatics are discussed.
Requirements for enrolment:
Inscription to adequate academic year. The student should already have attended the elementary Bbochemistry and molecular biology courses.
Terms of Prerequisites:
The successfully defended essay is required to take the exam.