Development of molecular genetic diagnostic methods

BT242

Biotehnology

Lecturer: Tanja Kunej

2. semester

Lectures 30 hours

Seminars 10

Seminar tutorials

Laboratory tutorials 10

Field work

Other work 10

Credit points 3 cp

Lectures

1. Main steps in development of molecular genetic tests.

2. Next generation sequencing (NGS): the importance in development of molecular markers associated with phenotypic traits and diseases.

3. Next generation sequencing: the importance for development of molecular genetic tests in personalized diagnostics.

4. Microarray technology in diagnostics

5. Presentation of steps in genetic diagnostic test development on cases in rare diseases.

6. The use of liquid biopsy for molecular genetic diagnostics.

7. Main databases and bioinformatics tools used for development of molecular genetics tests.

8. Examples of development of molecular genetics methods for disease diagnostics in animals and plants.

Seminar, exercises, other types of study

1. Ethical, legal and social aspects of genetic testing

2. NGS applications: practical examples

3. Microarray applications: practical examples

Prerequisite for the final exam: completed all obligations in the program, lectures, seminar and laboratory work.